European Sleep Research Laboratories

Sleep Team Helsinki

University of Helsinki
Institute of Biomedicine

PhD,MD
Tarja Porkka-Heiskanen (Stenberg)
PO Box 63
00014 Univ Helsinki Finland


Position Team Leader Phone +358 9 191 25317 Fax +358 9 191 25302 Contact E-Mail tarja.stenberg@helsinki.fi   Research Domain
  • Basic Animal

Keywords
  • Sleep Physiology-Neurophysiology in Animal
  • Sleep Physiology-Neurophysiology in Human
  • Sleep and Body Functions (Cardio-Respiratory, Thermoregulation/Metabolism, Endocrine)
  • Sleep and Ageing
  • Genetics of Sleep

Laboratory Description The main research topic is the molecular mechanisms of (recovery) sleep. We do research also on the effects of sleep deprivation on (human) health. Sleep and aging is a long-term topic of interest, as well as sleep and depression. Most research is conducted with animal models.
We use in vivo microdialysis, EEG recordings including spectral analysis, behavioral analyses.
We use animal models (mice, rats, zebra fish) - we have validated a clomipramine model of depression in rats and work on shift work models in zebra fish.

Technical Capabilities and areas of expertise In vivo microdialysis combined with HPLC analysis. Analysis, of adenosine, lactate, nitric oxide, histamine, monoamines. EEG in rodents. Movement/behavioral analysis in zebra fish. Sleep deprivations in rodents and zebra fish. Behavioral analyses in rodents.
Additional Descriptors, Keywords Metabonomics.
Selected Publications in the 5 past years Van Leeuwen, W.M.A., Lehto,M, Kaarisola, P, Lindholm, H., Sallinen, M., Härmä, M., Porkka-Heiskanen, T., Alenius, H., Sleep restriction increases the risk of cardiovascular diseases by enhancing proinflammatory responses through IL-17 and CRP. PloS ONE 4(2): e4589, 2009

Wigren, H-K, Porkka-Heiskanen, T., Inducible nitric oxide synthase and AMP activated protein kinase in the rat basal forebrain during prolonged waking. Neuroreport 7;20(1):97-101, 2009

Gass, N, Porkka-Heiskanen, T, Kalinchuk, A, The role of basal forebrain adenosine receptors in sleep homeostasis. Neuroreport 20(11): 1013-8, 2009

Paavonen, J.E., Porkka-Heiskanen, T., Lahikainen, A.R., Sleep quality, duration and behavioral symptoms among 5-6-year-old children. European Child & Adolescent Psychiatry

Paavonen, J.E., Räikkönen, K., Lahti, J., Komsi, N., Heinonen, K., Pesonen, A-K., Järvenpää, A-L., Timo Strandberg, T., Kajantie, E., Porkka-Heiskanen, T., Short sleep and behavioural symptoms of ADHD in healthy 7-8-year-old children. Pediatrics 123 (5):e857-64, 2009

Wigren, H-K, Rytkönen, K-M, Porkka-Heiskanen, T, Aging attenautes basal forebrain lactate release and promotion of cortical arousal during prolonged wakefulness J Neurosci, 16;29(37):11698-707, 2009

Utge, S, Soronen, P, Partonen, T., Loukola, A., Kronholm,E., Pirkola, S., Kiviruusu, O., Nyman, E.,
Porkka-Heiskanen, T., Paunio, T., A population-based association study of mood genes in depression and sleep disturbance. Am J Med Genet B Neuropsychiatric Genetics

Mäkelä, K.A. Wigren, H-K., Zant, J.C., Sakurai,T., Alhonen, L., Kostin,A., Porkka-Heiskanen, T., Herzig K-H., Characterization of sleep-wake patterns in a novel transgenic mouse line overexpressing human prepro-orexin. Acta Physiologica Scandinavia 198(3): 237-249, 2010

Kostin, A, Stenberg, D, Porkka-Heiskanen, T, Effect of sleep deprivation on multiunit discharge activity of basal forebrain. J Sleep Res 19(2):269-279, 2010

Paavonen, E.J., Räikkönen, K., Pesonen, A-K., Lahti,J., Komsi, N., Heinonen, K., Järvenpää, A-L.,Strandberg, T., Kajantie, E., Porkka-Heiskanen, T., Sleep quality and cognitive performance in 8 year old children. Sleep Medicine 11(4):386-392, 2010

Kalinchuk, A., McCarley RW, Porkka-Heiskanen, T., Basheer, R., Sleep deprivation triggers inducible nitric oxide - dependent nitric oxide production in wake-active basal forebrain neurons.
J Neurosci 30 (40): 13254-13264, 2010

Rytkönen, K-M., Wigren, H-K., Kostin, A., Porkka-Heiskanen, T, Kalinchuk, A., Nitric oxide mediated recovery sleep is attenuated with aging. Neurobiol Aging, 31 (11): 2011 – 2019, 2010

Haavisto M-L, Porkka-Heiskanen T, Hublin C, Härmä M, Mutanen P, Müller K, Virkkala J, Sallinen M, Sleep Restriction for the Duration of a Work Week Impairs Multitasking Performance J Sleep Res 19: 444-454, 2010

Stenholm,S., Kronholm,E., Borodulin,K., Era,P., Fogelholm,M., Partonen, T., Sainio,P., Porkka-Heiskanen, T., Koskinen, S., Sleep-related factors and mobility limitation in older men and women.,J Gerontol 65(6): 649-657, 2010

Utge,S., Soronen, P., Loukola,A., Kronholm,E., Ollila, H., Pirkola,S., Porkka-Heiskanen,T., Partonen,T., Paunio,T., Systematic analysis of circadian genes in a population-based sample reveals association of TIMELESS with depression and sleep disturbance PLoS ONE 5(2) e9259, 2010

Lagus, M., Gass, N., Saharinen, J., Saarela, J., Porkka-Heiskanen, T., Paunio, T., Gene expression patterns in a rodent model for depression. Eur J Neuroscience 31: 1465-1473, 2010

Van Leeuwen, W.M.A, Hublin, C, Sallinen, M, Härmä, M, Hirvonen, A. Porkka-Heiskanen, T, Prolonged sleep restriction affects glucose metabolism in healthy young men. Int J Endocrinol 2010,

Gass N, Ollila HM, Utge S, Partonen T, Kronholm E, Pirkola S, Suhonen J, Silander K, Porkka-Heiskanen T, Paunio T. Contribution of adenosine related genes to the risk of depression with disturbed sleep. Journal of Affective Disorders 126 (1-2): 134-139, 2010

Kalinchuk,A., McCarley, RW, Porkka-Heiskanen, T., Basheer, R, The time course of adenosine, nitric oxide (NO) and inducible NO synthase changes in the brain with sleep loss and their role in the NREM sleep homeostatic cascade J Neurochem 116(2): 260-272, 2011

Utge, S, Kronholm,E, Partonen, T, Soronen,P, Loukola, A, Porkka-Heiskanen, T, Tiina Paunio, T,
Shared Genetic Background for Short or Long Sleeper and Depression., Sleep 34(10):1309-16, 2011

Zant JC, Leenaars CHC, Kostin A, Van Someren EJW, Porkka-Heiskanen T, Sleep deprivation increases serotonin and dopamine turnover, and corticosterone in the basal forebrain.
Brain Res 1399:40-8, 2011

Rytkönen, K-M, Zitting, J, Porkka-Heiskanen, T, Automated sleep scoring in rats and mice using the naive Bayes classifier. J Neurosci Methods 202(1):60-4, 2011

Savelyev SA, Rantamäki T, Rytkönen KM, Castren E, Porkka-Heiskanen T.,Sleep homeostasis and depression: studies with the rat clomipramine model of depression.Neuroscience. 2012 Jun 14;212:149-58.

Lagus M, Gass N, Saharinen J, Savelyev S, Porkka-Heiskanen T, Paunio T.,Inter-tissue networks between the basal forebrain, hippocampus, and prefrontal cortex in a model for depression caused by disturbed sleep. J Neurogenet. 2012: Sep;26(3-4):397-412

Ollila HM, Utge S, Kronholm E, Aho V, Van Leeuwen W, Silander K, Partonen T, Perola M, Kaprio J, Salomaa V, Sallinen M, Härmä M, Porkka-Heiskanen T, Paunio T.,TRIB1 constitutes a molecular link between regulation of sleep and lipid metabolism in humans.,Transl Psychiatry. 2012 Mar 20;2:e97.

Zant JC, Rozov S, Wigren HK, Panula P, Porkka-Heiskanen T.,Histamine release in the basal forebrain mediates cortical activation through cholinergic neurons., J Neurosci. 2012 Sep 19;32(38):13244-54.

Sallinen M, Onninen J, Tirkkonen K, Haavisto ML, Härmä M, Kubo T, Mutanen P, Virkkala J, Tolvanen A, Porkka-Heiskanen T.,Effects of cumulative sleep restriction on self-perceptions while multitasking.J Sleep Res. 2013 Jun;22(3):273-81

Aho V, Ollila HM, Rantanen V, Kronholm E, Surakka I, van Leeuwen WM, Lehto M, Matikainen S, Ripatti S, Härmä M, Sallinen M, Salomaa V, Jauhiainen M, Alenius H, Paunio T, Porkka-Heiskanen T., Partial sleep restriction activates immune response-related gene expression pathways: experimental and epidemiological studies in humans.PLoS One. 2013 Oct 23;8(10):e77184.


Key Publications of the Laboratory Porkka-Heiskanen, T., Strecker,R.E., Bjorkum, A.A., Thakkar, M., Greene,R.W., McCarley, R.W., Adenosine: A mediator of the sleep-inducing effects of prolonged wakefulness.
Science 276: 1265-1268, 1997

Porkka-Heiskanen, T., Strecker, R.E., McCarley,R.W., Brain site specificity of extracellular adenosine concentration changes during sleep deprivation and spontaneous sleep: an in vivo microdialysis study.Neuroscience 99 (3): 507-517, 2000

Urrila, A.S., Hakkarainen, Heikkinen, S.,Vuori, K., Stenberg, D., A., Häkkinen, A-M., Lundbom, N., Porkka-Heiskanen, T., Metabolic imaging of human cognition: an fMRI/ 1H MRS study of brain lactate response to silent word generation. J Cereb Blood Flow and Metab 23(8): 942-948, 2003 (5.477).

Stenberg, D., Litonius, E., Linda Halldner, Johansson, B., Fredholm, B.B., Porkka-Heiskanen, T.,
Sleep and its homeostatic regulation in mice lacking the adenosine A1-receptor. J Sleep Res, 12 (4):283-290, 2003 (3.14)

Porkka-Heiskanen, T., Alanko,L., Kalinchuk, A., Heiskanen, S., Stenberg, D., The effect of age on pre-pro orexin gene expression and contents of orexin A and B in the rat brain. Neurobiology of ageing 25:231-238, 2004 (4.49).

Urrila, A.S., Hakkarainen, A., Heikkinen, S., Vuori, K., Stenberg, D., Häkkinen, A-M., Lundbom, N., Porkka-Heiskanen, T., Stimulus-induced brain lactate: effects of aging and prolonged wakefulness.
J Sleep Res 13:111-119, 2004

Urrila, A.S., Hakkarainen, A., Huhdankoski, O., Heikkinen, S., Kuusi, T., Stenberg, D., Häkkinen, A.M., Porkka-Heiskanen, T., Lundbom, N., Preliminary findings of proton magnetic resonance spectroscopy in occipital cortex during sleep deprivation. Psychiatry Research: Neuroimaging 147: 41-46, 2006.

Kalinchuk,A., Lu,Y., Stenberg,D., Rosenberg,P.A., Porkka-Heiskanen,T., Nitric oxide production in the basal forebrain is required for recovery sleep. J Neurochem. 99:483-498, 2006

Kalinchuk, A., Stenberg,D., Rosenberg, P., Porkka-Heiskanen,T., Inducible and neuronal nitric oxide synthases (NOS) have complementary roles in recovery sleep induction. Eur J Neurosci 24:1443-1456, 2006

Wigren, H-K., Schepens,M., Matto,V., Stenberg,D., Porkka-Heiskanen,T., Glutamatergic stimulation of the basal forebrain in vivo elevates extracellular adenosine concnetration and increases subsequent NREM sleep. Neuroscience 147: 811-823, 2007

Kostin, A., Stenberg, D., Kalinchuk, A.V., Porkka-Heiskanen, T., Nitric oxide modulates the discharge rate of basal forebrain neurons. Psychopharmacology 201: 147-60, 2008

Kalleinen, N., Polo-Kantola,P., Irjala, K., Porkka-Heiskanen, T., Vahlberg,T., Virkki, A., Polo, O.
24-h serum levels of growth hormone, prolactin and cortisol, in pre- and postmenopausal women. The effect of combined estrogen and progestin treatment. J Clin Endocrin Metab 93:1655-61, 2008.

Kalinchuk, A.V., McCarley, R.W., Stenberg, D., Porkka-Heiskanen, T., Basheer, R, The role of cholinergic basal forebrain neurons in adenosine-mediated homeostatic control of sleep: lessons from 192 IgG saporin lesions. Neuroscience 157: 238–253, 2008

Kostin,A, Stenberg D, Porkka-Heiskanen T., Nitric oxide modulates the discharge rate of basal forebrain neurons. Psychopharmacology 201(1): 147-60, 2008

Van Leeuwen, W.M.A., Lehto,M, Kaarisola, P, Lindholm, H., Sallinen, M., Härmä, M., Porkka-Heiskanen, T., Alenius, H., Sleep restriction increases the risk of cardiovascular diseases by enhancing proinflammatory responses through IL-17 and CRP. PloS ONE 4(2): e4589, 2009

Wigren, H-K, Porkka-Heiskanen, T., Inducible nitric oxide synthase and AMP activated protein kinase in the rat basal forebrain during prolonged waking. Neuroreport 7;20(1):97-101, 2009

Gass, N, Porkka-Heiskanen, T, Kalinchuk, A, The role of basal forebrain adenosine receptors in sleep homeostasis. Neuroreport 20(11): 1013-8, 2009

Wigren, H-K, Rytkönen, K-M, Porkka-Heiskanen, T, Aging attenautes basal forebrain lactate release and promotion of cortical arousal during prolonged wakefulness J Neurosci, 16;29(37):11698-707, 2009

Utge, S, Soronen, P, Partonen, T., Loukola, A., Kronholm,E., Pirkola, S., Kiviruusu, O., Nyman, E.,
Porkka-Heiskanen, T., Paunio, T., A population-based association study of mood genes in depression and sleep disturbance. Am J Med Genet B Neuropsychiatric Genetics DOI 10.1002/ajmg.b.31002, 2010

Kostin, A, Stenberg, D, Porkka-Heiskanen, T, Effect of sleep deprivation on multiunit discharge activity of basal forebrain. J Sleep Res 19(2):269-279, 2010

Kalinchuk, A., McCarley RW, Porkka-Heiskanen, T., Basheer, R., Sleep deprivation triggers inducible nitric oxide - dependent nitric oxide production in wake-active basal forebrain neurons.
J Neurosci 30 (40): 13254-13264, 2010

Rytkönen, K-M., Wigren, H-K., Kostin, A., Porkka-Heiskanen, T, Kalinchuk, A., Nitric oxide mediated recovery sleep is attenuated with aging. Neurobiol Aging, 31 (11): 2011 – 2019, 2010

Haavisto M-L, Porkka-Heiskanen T, Hublin C, Härmä M, Mutanen P, Müller K, Virkkala J, Sallinen M, Sleep Restriction for the Duration of a Work Week Impairs Multitasking Performance J Sleep Res 19: 444-454, 2010

Stenholm,S., Kronholm,E., Borodulin,K., Era,P., Fogelholm,M., Partonen, T., Sainio,P., Porkka-Heiskanen, T., Koskinen, S., Sleep-related factors and mobility limitation in older men and women
J Gerontol 65(6): 649-657, 2010

Utge,S., Soronen, P., Loukola,A., Kronholm,E., Ollila, H., Pirkola,S., Porkka-Heiskanen,T., Partonen,T., Paunio,T., Systematic analysis of circadian genes in a population-based sample reveals association of TIMELESS with depression and sleep disturbance PLoS ONE 5(2) e9259, 2010

Lagus, M., Gass, N., Saharinen, J., Saarela, J., Porkka-Heiskanen, T., Paunio, T., Gene expression patterns in a rodent model for depression. Eur J Neuroscience 31: 1465-1473, 2010

Van Leeuwen, W.M.A, Hublin, C, Sallinen, M, Härmä, M, Hirvonen, A. Porkka-Heiskanen, T, Prolonged sleep restriction affects glucose metabolism in healthy young men. Int J Endocrinol 2010, ID 108641, doi: 10.1155/2010/108641

Gass N, Ollila HM, Utge S, Partonen T, Kronholm E, Pirkola S, Suhonen J, Silander K, Porkka-Heiskanen T, Paunio T. Contribution of adenosine related genes to the risk of depression with disturbed sleep. Journal of Affective Disorders 126 (1-2): 134-139, 2010

Rytkönen, K-M, Zitting, J, Porkka-Heiskanen, T, Automated sleep scoring in rats and mice using the naive Bayes classifier. J Neurosci Methods 2011

 
Other laboratory Members
 
Title MSc First Name Vilma Last Name Aho Position PhD student E-Mail vilma.aho@helsinki.fi  
Title MSc First Name Anu Last Name Lintulahti Position PhD student E-Mail anu.lintulahti@helsinki.fi  
First Name Eeva Last Name Palomäki Position PhD student E-Mail eeva.palomaki@helsinki.fi  
First Name Sonja Last Name Sulkava Position PhD student E-Mail sonja.sulkava@helsinki.fi  
Title PhD First Name Henna-Kaisa Last Name Wigren Position postdoctoral fellow E-Mail henna-kaisa.wigren@helsinki.fi  
Title BSc First Name Pirjo Last Name Saarelainen Position Medical laboratory technologist E-Mail pirjo.saarelainen@helsinki.fi  
Collaborations
 
 
Title PhD,MD First Name Tiina Last Name Paunio Position professor, P.I. Institution National Institute for Health and Welfare and University of Helsinki  
Past Members
 
Title PhD First Name Anna Last Name Kalinchuk Position Instructor of Psychiatry, Harvard Medical School E-Mail anna_Kalinchuk@hms.harvard.edu  
Title PhD,MD First Name Anna Last Name Urrila Position adolescent psychiatrist, Helsinki University Central Hospital, Department of Psychiatry E-Mail anna.urrila@helsinki.fi  
First Name Janneke Last Name Zant Position Research Fellow in Psychiatry (INT), Harward Medical School E-Mail janneke_Zant@hms.harvard.edu  
First Name Hanna Last Name Ollila Position Post doctoral fellow, Stanford University E-Mail hannao@stanford.edu  
Title PhD First Name Kirsi-Marja Last Name Zitting (Rytkönen) Position Post doctoral fellow, Harward Medical school E-Mail kirsimarja.rytkonen@gmail.com