European Sleep Research Laboratories

Physiopathology of the neuronal networks of the sl

UMR 5292 CNRS/U1028 INSERM
Team "Sleep"

PhD
Pierre-Hervé Luppi
7, Rue Guillaume Paradin
69372 Lyon France


Position DR1 CNRS Phone +33478771040 Fax +33478771022 Contact E-Mail luppi@sommeil.univ-lyon1.fr Website http://crnl.univ-lyon1.fr/index.php/fr/Recherche/Equipes/7   Research Domain
  • Basic Animal

Keywords
  • Molecular Biology-Endocrinology-Biochemistry
  • Learning, Memory, Cognition
  • Pharmacology of Sleep
  • Sleep Physiology-Neurophysiology in Animal
  • Behavioural Sleep Disorders
  • Neurological Sleep Disorders

Laboratory Description 11 INSERM/CNRS researchers,
1 University researcher,
7 technical staff,
11 PhD students
Areas of research:

• Neuronal mechanisms of the sleep-wake cycle: identification of brain structures and their interconnections involved in the genesis, maintenance and homeostasis of sleep, especially paradoxical (REM) sleep in normal conditions and animal models of sleep disorders.
• The function of sleep, particularly the role of paradoxical sleep in memory consolidation: multi-level (behavior, network, cellular) investigation of the beneficial role of sleep in learning and memory.
• The impact of sleep on mood regulation and depression.

Technical Capabilities and areas of expertise Electrophysiology (in vivo and in vitro): EEG, LFP, single-unit recordings, multi-electrode recordings in freely-moving /head-restrained rodents, intracellular recordings, patch-clamp
Polysomnography, Pharmacology, optogenetics in freely moving rodents
Behavioral learning and memory paradigms, selective sleep deprivations
Tract-tracing studies, functional neuroanatomy, immunochemistry, in situ hybridization, transcriptomics
Transgenic mice, basic biochemical and molecular techniques.

Additional Descriptors, Keywords • Brain mechanisms underlying PS onset, maintenance and homeostasis (P.H. Luppi, P. Fort, D. Gervasoni)
• Brainstem mechanisms of PS atonia and REM-behavior disorder (P.H. Luppi, P. Fort)
• Hypothalamic regulation of paradoxical sleep and narcolepsy (C. Peyron, P. Fort)
• Role of sleep in learning and memory, hippocampal sleep-dependent plasticity (G. Malleret, P.Salin, R.Parmentier)
• Physio-pathology of sleep and learning in an animal model of depression (J-M Vaugeois, M. El Yacoubi)
• Anatomical substrate of depression and addiction (A.Bérod)

Selected Publications in the 5 past years 2006

58- VERRET L., FORT P., LEGER L. and LUPPI P-H. : Localization of the neurons responsible for inhibition of Locus Coeruleus noradrenergic neurons during paradoxical sleep . J. Comp. Neurol, 2006, 495 : 573-586.

59- COMTE J.C., SCHATZMAN M., RAVASSARD P., LUPPI P-H., SALIN P., “A Three states sleep-waking model”, Chaos, Solitons & Fractals, 2006, 29: 808-815.
.
60- MORALES F.R., SAMPOGNA S., RAMPON C., LUPPI P-H., CHASE M.H., Brainstem glycinergic neurons and their activation during active (rapid eye movement) sleep in the cat. Neuroscience, 2006, 142(1) : 37-47

2007

61- LUPPI P-H, DAMIEN GERVASONI D., LAURE VERRET L., GOUTAGNY R., PEYRON C., SALVERT D., LEGER L. ASND FORT P.: Paradoxical (REM) sleep genesis: the switch from an aminergic-cholinergic to a GABAergic-glutamatergic hypothesis. J. Physiol. Paris, 2007, 100: 271-283.

62- HANRIOT L., CAMARGO N., COURAU A.C., LEGER L., LUPPI P-H and PEYRON C.: Characterization of the melanin concentrating hormone neurons activated during paradoxical sleep hypersomnia in rat. J. Comp. Neurol., 2007, 505, 147-157.

2008

63- GOUTAGNY R., LUPPI P-H, SALVERT D., LAPRAY D.; GERVASONI D. AND PATRICE FORT P.: Role of the dorsal paragigantocellular reticular nucleus in the inhibition of the wake-related neurons during paradoxical (REM) sleep in rat. Neuroscience. 2008, 152, 849-857.

64- ADAMANTIDIS A., SALVERT D. , GOUTAGNY R., , LAKAYE B., GERVASONI D. , GRISAR T., LUPPI P-H. and FORT P. Sleep architecture of the melanin-concentrating hormone receptor 1-knockout mice. European Journal of Neuroscience 2008, 7:1793-800.
65- ALBOUY G, STERPENICH V, BALTEAU E, VANDEWALLE G, DESSEILLES M, DANG-VU T, DARSAUD A, RUBY P, LUPPI PH, DEGUELDRE C, PEIGNEUX P, LUXEN A, MAQUET P. Both the hippocampus and striatum are involved in consolidation of motor sequence memory. Neuron. 2008, 58:261-72.
66- FORT P, SALVERT D, HANRIOT L, JEGO S, SHIMIZU H, HASHIMOTO K, MORI M, LUPPI PH. The satiety molecule nesfatin-1 is co-expressed with melanin concentrating hormone in tuberal hypothalamic neurons of the rat. Neuroscience. 2008;155:174-81.

2009

67- SAPIN E, LAPRAY D, BEROD A, GOUTAGNY R, LEGER L, RAVASSARD P, CLEMENT O, HANRIOT L, FORT P, LUPPI PH. Localization of the brainstem GABAergic neurons controlling paradoxical (REM) sleep. PLoS ONE. 2009; 4:e4272. 2009
68- FORT P., BASSETI C. and LUPPI P-H. : Alternating vigilance states : new insights regarding neuronal networks and mechanisms. Eur J Neurosci, 2009, 29: 1741-53.
69- LEGER L., GOUTAGNY R., SAPIN E., SALVERT D., FORT P. and LUPPI P-H. : Noradrenergic neurons expressing Fos during waking and paradoxical sleep deprivation in the rat. J Chem Neuroanat, 2009, 37 (3): 149-57.
70- RAVASSARD P., PACHOUD B., COMTE J-C., MEJIA-PEREZ C., SCOTE-BLACHON C., GAY N., CLAUSTRAT B., TOURET M., LUPPI P-H. and SALIN P.A. : Paradoxical (REM) sleep deprivation causes a large and rapidly reversible decrease in long-term potentiation, synaptic transmission, glutamate receptor protein levels, and ERK/MAPK activation in the dorsal hippocampus. Sleep, 2009, 32 : 227-40.
71- BAYKUT S., GONÇALVES P., LUPPI P-H., ABRY P., PEREIRA DE SOUZA NETO E. and GERVASONI D. : EMD-based analysis of rat EEG data for sleep state classification. Biosignals, 2009 115-123.
72- PEYRON C., SAPIN E., LEGER L., LUPPI P-H. and FORT P. : Role of the Melanin-Concentrating Hormone neuropeptide in sleep regulation. Peptides, 2009 Nov;30(11):2052-9.
73- SAPIN E., LEGER L., BEROD A., HERMAN P.A., LUPPI P-H. and PEYRON C. : Evidence that the tuberal hypothalamus contains a very large number of GABAergic neurons specifically active during paradoxical (REM) sleep. PLoS One. 2009;4(1):e4272.

2010

74- PACHOUD B, ADAMANTIDIS A, RAVASSARD P, LUPPI PH, GRISAR T, LAKAYE B, SALIN PA : Major impairments of glutamatergic transmission and long-term synaptic plasticity in the hippocampus of mice lacking the melanin-concentrating hormone receptor-1. J Neurophysiol. 2010 Sep;104(3):1417-25.
75- SAPIN E, BEROD A, LEGER L, HERMAN PA, LUPPI PH, PEYRON C. A very large number of GABAergic neurons are activated in the tuberal hypothalamus during paradoxical (REM) sleep hypersomnia. PLoS One. 2010 Jul 26;5(7):e11766.
LEGER L, SAPIN E, GOUTAGNY R, PEYRON C, SALVERT D, FORT P, LUPPI P LUPPI PH. Dopaminergic neurons expressing Fos during waking and paradoxical sleep in the rat. J Chem Neuroanat. 2010 Jul; 39(4):262-71.
76- LUPPI PH. Neurochemical aspects of sleep regulation with specific focus on
slow-wave sleep. World J Biol Psychiatry. 2010 Jun;11 Suppl 1:4-8.

2011


77- PEYRON C, VALENTIN F, BAYARD S, HANRIOT L, BEDETTI C, ROUSSET B, LUPPI PH, DAUVILLIERS Y. Melanin concentrating hormone in central hypersomnia. Sleep Med.2011 Sep;12(8):768-72. Epub 2011 Jun 22.
78- LUPPI PH, CLEMENT O, SAPIN E, GERVASONI D, PEYRON C, LEGER L, SALVERT D, FORT P. The neuronal network responsible for paradoxical sleep and its dysfunctions causing narcolepsy and rapid eye movement (REM) behavior disorder. Sleep Med Rev.2011 Jun;15(3):153-63.
79- CLEMENT O, SAPIN E, BEROD A, FORT P, LUPPI PH. Evidence that neurons of the
sublaterodorsal tegmental nucleus triggering paradoxical (REM) sleep are glutamatergic. Sleep. 2011 Apr 1;34(4):419-23.
80- LUPPI PH, FORT P. Neurochemistry of sleep an overview of animal experimental
work. Handb Clin Neurol. 2011;98:173-90.
81- LUPPI P-H. and FORT P. : What are the mechanisms activating the sleep-active neurons located in the preoptic area. Sleep and Biological Rhythms, 2011, 9 : 59-64.

2012

82- LUPPI PH, CLEMENT O, SAPIN E, PEYRON C, GERVASONI D, LEGER L, FORT P. Brainstem mechanisms of paradoxical (REM) sleep generation. Pflugers Arch. 2012
Jan;463(1):43-52.
83- SIRIEIX C, GERVASONI D, LUPPI PH, LEGER L. Role of the lateral paragigantocellular nucleus in the network of paradoxical (REM) sleep: an electrophysiological and anatomical study in the rat. PLoS One. 2012;7(1):e28724.

 
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